Electroporation Protocols

Zusatztext

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.

Autorenportrait

InhaltsangabePart I. Basic Concepts and Enhancement of DNA Transfer via Electroporation  1. Electroporation-Based Gene Therapy: Recent Evolution in the Mechanism Description and Technology DevelopmentsLluis M. Mir 2. Electropermeabilization of the Cell MembraneJustin Teissié 3. The Impact of Non-Electrical Factors on Electrical Gene TransferJiemiao Hu, Jeffry Cutrera, and Shulin Li 4. Electroporation Formulation for Cell TherapyJiemiao Hu and Shulin Li 5. Short-Fragment DNA-Mediated in Vivo DNA Electroporation Delivery Jinliang Peng, Yonggang Zhao, and Yuhong Xu 6. Gold Nanoparticle-Enhanced Electroporation for Leukemia Cell TransfectionShuyan Huang, Yingbo Zu, and Shengnian Wang  Part II. SiRNA Electrotransfer 7. SiRNA Delivery via Electropulsation: A Review of the Basic ProcessesMuriel Golzio and Justin Teissié 8. Continuous Cell Electroporation for Efficient DNA and siRNA Delivery Based on Laminar Microfluidic ChipsZewen Wei and Zhihong Li 9. Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic CellsIsabel Popp, Valentin Schatz, Alexander T. Prechtel, Alexander Steinkasserer, Christian Bogdan, and Jonathan Jantsch 10. Direct Imaging of siRNA Electrotransfer at the Single Cell LevelJustin Teissié and Muriel Golzio 11. Electroporation-Mediated siRNA Delivery into TumorsYoshifumi Takei 12. Substrate-Mediated, High Efficiency siRNA ElectroporationHiroyuki Fujimoto and Hiroo Iwata 13. Intradermal Electroporation of RNAMaria L. Knudsen, Karl Ljungberg, Peter Liljeström, and Daniel X. Johansson  Part III. Electroporation-Mediated Delivery to Poorly Accessible and Fragile Tissues 14. Delivery of DNA into the Central Nervous System via Electroporation Jochen De Vry, Tim Vanmierlo, Pilar Martínez-Martínez, Mario Losen, Yasin Temel, Harry Steinbusch, Marc De Baets, and Jos Prickaerts 15. DNA Delivery in Adult Mouse Eyes: An Update with Corneal OutcomesJohn M. Nickerson, Shannon E. Getz, Jana T. Sellers, Micah A. Chrenek, Penny Goodman, Christiana J. Bernal, and Jeffrey H. Boatright 16. Delivery of Plasmid DNA into Developing Rat Teeth by ElectroporationShaomian Yao, Michael L. Beckley, and Dawen Liu 17. Electroporation-Mediated Gene Delivery to the LungsJennifer L. Young, Michael S. Barravecchia, and David A. Dean 18. Electroporation-Mediated Delivery of Genes in Rodent Models of Lung ContusionDavid Machado-Aranda and Krishnan Raghavendran 19. In Vivo Electroporation-Mediated Gene Delivery to the Beating HeartHendrik T. Tevaearai, Amiq Gazdhar, M. N. Giraud, and Martin Flück   Part IV. Treatment of Cancer in Large Animals and Humans via Electroporation Therapy 20. Managing Local Swelling Following Intratumoral Electro-Chemo-Gene TherapyJeffry Cutrera, Glenn King, Pamela Jones, Elias Gumpel, Xueqing Xia, and Shulin Li 21. Gene Electrotransfer in Clinical TrialsJulie Gehl 22. Electrochemotherapy in Veterinary Oncology: From Rescue to First-line TherapyEnrico P. Spugnini and Alfonso Baldi 23. Low Energy DC Current Ablation in a Mouse Tumor ModelPrejesh Philips and Robert C. G. Martin  Part V. Applications of Electroporation for DNA Vaccination 24. Evolution of Electroporated DNA VaccinesAndrea Keane-Myers and Matthew Bell 25. Clinical Development of Intramuscular Electroporation: Providing a "Boost" for DNA VaccinesAmir S. Khan, Kate E. Broderick, and Niranjan Y. Sardesai 26. Electroporation-Mediated Adminis

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