Parasite Genomics Protocols

Zusatztext

Parasite Genomics Protocols, Second Edition expands upon the previous edition with current, detailed methodologies that have been adapted to research parasites in the post genomic era. With new chapters on parasite studies, helminth pathogens, and protocols for undertaking other large-scale 'omics' methodologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Parasite Genomics Protocols, Second Edition offers a wide-ranging collection of diverse methods and protocols for further study into these unique organisms.

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Inhaltsangabe1. The Eukaryotic Pathogen Databases: A Functional Genomic Resource Integrating Data from of Human and Veterinary ParasitesOmar S. Harb and David S. Roos  2. From Sequence Mapping to Genome AssembliesThomas D. Otto 3. Sequencing and Annotation of Mitochondrial Genomes from Individual Parasitic HelminthsAaron R. Jex, D. Timothy Littlewood, and Robin B. Gasser 4. A Beginners Guide to Estimating the Non-synonymous to Synonymous Rate Ratio of all Protein-coding Genes in a GenomeDaniel C. Jeffares, Bartlomiej Tomiczek,Victor Sojo, and Mario dos Reis 5. Exploiting Genetic Variation to Discover Genes Involved in Important Disease PhenotypesPaul Capewell, Anneli Cooper, Caroline Clucas, Willie Weir, Heli Vaikkinen, Liam Morrison, Andy Tait, and Annette MacLeod  6. Identification and Analysis of Ingi-Related Retroposons in the Trypanosomatid GenomesFrédéric Bringaud, Matthew Rogers, and Elodie Ghedin 7. Approaches for Studying mRNA Decay Mediated by SIDER2 Retroposons in LeishmaniaBarbara Papadopoulou, Michaela Müller-McNicoll, and Prasad K. Padmanabhan 8. Gene Suppression in Schistosomes using RNAiAkram  A. Da'dara  and  Patrick  J.  Skelly 9. Construction of Trypanosoma brucei Illumina RNA-Seq Libraries Enriched for Transcript EndsNikolay G. Kolev, Elisabetta Ullu and Christian Tschudi 10. Techniques to Study Epigenetic Control and the Epigenome in Parasites Sheila C. Nardelli, Li-Min Ting, and Kami Kim 11. The Genome-wide Identification of Promoter Regions in Toxoplasma gondiiJunya Yamagish and Yutaka Suzuki 12. RNA-seq Approaches for Determining mRNA Abundance in LeishmaniaAndrew Haydock, Monica Terrao, Aarthi Sekar, Gowthaman Ramasamy, Loren Baugh, and Peter J. Myler13. Protein Microarrays for Parasite Antigen DiscoveryPatrick Driguez, Denise L Doolan, Douglas M Molina, Alex Loukas, Angela Trieu, Phil L Felgner, and Donald P McManus 14. A Transposon-based Tool for Transformation and Mutagenesis in Trypanosomatid ProtozoaJeziel D. Damasceno, Stephen M. Beverley, and Luiz R. O. Tosi 15. Separation of Basic Proteins from Leishmania using a combination of Free Flow Electrophoresis (FFE) and 2D Electrophoresis (2-DE) under Basic Conditions Marie-Christine Brotherton, Gina Racine, and Marc Ouellette 16. Proteomic Analysis of Post Translational Modifications using iTRAQ in LeishmaniaDan Zilberstein 17. Large Scale Differential Proteome Analysis in Plasmodium falciparum under Drug TreatmentJudith Helena Prieto, Elisabeth Fisher, Sasa Koncarevic, John Yates, and Katja Becker 18. Use of 13C Stable Isotope Labelling for Pathway and Metabolic Flux Analysis in Leishmania ParasitesEleanor C. Saunders, David P. de Souza, Jennifer M. Chambers, Milica Ng, James Pyke, and Malcolm J. McConville 19. Molecular Genotyping of Trypanosoma cruzi for Lineage Assignment and Population Genetics Louisa A. Messenger, Matthew Yeo, Michael D. Lewis, Martin S. Llewellyn, and Michael A. Miles  20. Screening Leishmania donovani Complex-specific Genes Required for Visceral DiseaseWen-Wei Zhang and Greg Matlashewski 

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